Ion channel involvement in hypoxia-induced spreading depression in hippocampal slices

Abstract

Listen

Translate article

Rat hippocampal tissue slices were made hypoxic in control medium and in medium containing the ion channel blockers tetraethylammonium (TEA), 4-aminopyridine (4-AP), or tetrodotoxin (TTX). Postsynaptic evoked potentials, extracellular DC potential V ec , and in some experiments extracellular potassium concentration [K +] o were monitored in stratum pyramidale of the CA1 region. TEA (10 mM) decreased the latency of hypoxia-induced spreading depression (SD), and reduced the amplitudes of the changes in V ec and [K +] o. 4-AP (50 μM) also decreased the latency of SD but had no effect on the V ec shift. In most slices, TTX (1 μM) increased SD latency but had no effect on the V ec shift. In some slices, TTX blocked the occurrence of SD.