Abstract
The ionizing radiation-induced release of a surface exonuclease from Micrococcus radiodurans is to a large extent inhibited by the removal of water. Irradiation of a cell suspension saturated with O$sub 2$ (an effective aqueous electron and hydrogen atom scavenger) allows the same release as irradiation in the presence of N$sub 2$. Ethanol (a good hydroxyl radical scavenger) protects the enzyme from release. These data suggest that hydroxyl radicals produced by the radiolysis of water are important releasing agents. Hydroxyl radicals produced by the ultraviolet decomposition of H$sub 2$O$sub 2$ were effective in releasing the enzyme. (auth)
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call