Investigation on Electrochemical Behavior and Scavenging Superoxide Anion Ability of Chrysin at Mercury Electrode

Abstract

AbstractThe electrochemical behavior of chrysin in pH 2.0‐9.0 Britton‐Robinson (B‐R) buffer solutions was studied by the means of linear sweep voltammetry and cyclic voltammetry at a static mercury drop electrode. In different pH range of B‐R buffer solutions, chrysin could cause four reduction waves. In pH 2.0‐5.8 B‐R buffer solutions, wave P1 yielded by chrysin is a one‐electron reduction wave, and wave P1′ caused by further reduction of the products of wave P1 in pH<3.0 B‐R buffer solution is also a one‐electron reduction wave. But in 3.0<pH<5.8 B‐R buffer solution wave P1′ was overlapped by the hydrogen wave. Between pH 5.8 and 9.0, chrysin could yield two reduction waves P2 and P3. The former is an irreversible adsorptive wave of ionized chrysin involving one electron and the latter is also an irreversible adsorptive wave of reduction intermediate radical of chrysin involving one electron and one proton. And a linear relationship between ip3 and the concentration of chrysin can be established from 1.0× 10‐6 to 4.0×10‐5 mol·L‐1 (r=0.9924) with the detection limit of 5×10‐7 mol·L‐1. In addition, the antioxidant ability of chrysin was investigated by linear sweep voltammetry (LSV). The determination result of IC50 of chrysin showed that chrysin is a good antioxidant.