Investigation on Voltammetric Behavior of Camptothecin and Its Analytical Application

Abstract

AbstractThe voltammetric behavior of camptothecin (CPT) in Britton‐Robinson (B‐R) buffer solutions (pH 2.09–9.07) was studied by the means of linear sweep voltammetry (LSV), cyclic voltammetry (CV) and normal pulse voltammetry (NPV) at a hanging mercury drop electrode. In different pH range of B‐R buffer solutions, CPT could cause three reduction waves. In B‐R buffer solutions (pH 2.09–5.46), wave P1 yielded by CPT was a two‐electron wave. Between pH 6.01 and 9.07, CPT could yield two reduction waves P2 and P3. In addition, the pure CPT obtained from camptotheca acumina grown only in China was determined by NPV, and a linear response was observed in the range of 2.0×10−3–4.0×10−2 mmol·L−1 with a 0.9991 correlation coefficient and a 8.0×10−4 mmol·L−1 detection limit for CPT.