Investigation of Yangzhou Region Blood Donors' ABO and Rh Blood Types Distribution and Correlation

Abstract

Objective To Explore Yangzhou blood donors' ABO and Rh blood groups distribution and correlation.Methods From January 2010 to June 2011,63925 samples collected by Yangzhou blood bank from unpaid blood donors on streets,were enrolled in this study.Positive and negative stereotypes of ABO blood specimens,identification and genotyping of Rh blood group were tested by U-shaped micro-plate methods.Rh negative confirmatory tests were recognized by anti-globulin method using three kinds IgG anti-D reagents from different manufacturers.Samples gene frequencies,haplotype frequencies and expected values were calculated.The phenotypic observed and expected value were compared by the X2 test,to test the Hardy-Weinberg goodness of fit.Results The gene frequencies of ABO blood group were r＞p＞q,phenotype distribution characteristics were O＞A＞B＞AB; Rh phenotypes population distribution were CCDee＞CcDEe＞CcDee＞ccDEE＞ccDEe＞CCDEe＞ccDee,ccdee＞Ccdee＞CCdee＞ccdEe＞CcdEe＞ccdEE,haplotype frequencies were CDe＞cDe＞cDE＞CDE,cde＞Cde＞cdE＞CdE,and gene frequencies characteristics were D＞d,C＞c,e＞E.The difference between the expected and observed values of the ABO blood group and Rh blood group were no statistical significance (P＞0.05).Hardy-Weinberg,the goodness of fit test results were good.Conclusions The distributions of ABO and Rh blood typing population in this region were in line with the Hardy-Weinberg population-based genetic equilibrium in population genetics.Through the survey of the ABO and Rh blood group distributions in Yangzhou region,we could accurately grasp the law of Yangzhou region blood donors' blood types distribution,which were contribute for unpaid blood donors recruitment,reasonable blood collection plan development,blood reserves and rare blood types establishment library. Key words: blood donor; ABO blood group; Rh blood group; population distribution; correlation