Abstract
A sensitive headspace solid phase micro-extraction gas chromatography/mass spectrometry analytical procedure was used to determine the furan content in blood and liver tissue. The method was employed to investigate in vivo metabolism leading to possible furan formation by feeding both 13C labeled and unlabeled (native) ascorbic acid, linoleic acid ethyl ester, or glucose to Fischer-344 rats. Rats were fed via oral intubation 0.16–0.96 g of a test compound in two 3 h intervals, and sacrificed 6 h later. The liver and blood tissues were collected and analyzed for the presence of furan. No significant formation of 13C labeled or native furan was observed from the substrates tested. Only minute traces attributable to 13C labeled furan were detected in blood and liver of rats fed ascorbic acid or linoleic acid ethyl ester but not glucose. Native furan was found in tissues of all animals tested. However, animals administered orally d4-furan at doses similar to those expected from the contribution of native furan in rat feed, showed no detectable levels of d4-furan. Data indicate the possibility that there is an endogenous formation of furan from substrates other than those tested in this study.
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