Abstract
This study investigated the stimulatory effect of hydrogen peroxide (H2O2) pre-treatment on cellular resistance to damage induced by ionizing radiation (IR), using Tetrahymena pyriformis as a model organism. The effects were evaluated on cell number, the activities of catalase (CAT) and superoxide dismutase (SOD), and lipid peroxidation. After 24 h of exposure to IR, cells pre-treated with H2O2 exhibited a marked improvement in cell viability, with increases of 42.2 and 58.5% for the 1-h and 24-h pre-treated cells, respectively, compared to the control cells without pre-treatment. Additionally, measurements of CAT and SOD activities revealed a significant decrease in pre-treated cells after irradiation compared to the control cells. Moreover, the level of malondialdehyde (MDA), a product of lipid peroxidation, decreased in pre-treated cells after irradiation, indicating a positive effect of H2O2 pre-treatment on the resistance of T. pyriformis cells to IR-induced damage.
Published Version
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