Abstract

Amsonia orientalis Decne. (Apocynaceae) is a medically useful and critically endangered plant which has very restricted distribution world-wide. The root tips and flower buds of A. orientalis were used as an experimental material and they were squashed in aceto-orcein. Fresh flowers and a liquid medium were used for pollen germination tests. In spite of the small size of the cells and chromosomes, all phases of mitosis and meiosis were observed. Generally the phases of mitosis were regular but there were a few abnormalities, such as laggard chromosome in metaphase and bridge formation in anaphase. There was a good relationship between the stages of pollen development and floral bud length. As a result of the cytokinesis, tetrahedral types of tetrads were occurred in microsporogenesis. The tetrad nuclei resulting from the simultaneous type of meiosis were found to be of equal size. Pollen germination had started from the first hour and pollen tube lengths and germination percentages regularly increased wit...

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