Abstract
Objectives: This study aimed to isolate the polyphenolic constituents from the methanol extract of Livistona decipiens Becc leaves and evaluate the antioxidant, cytotoxic and antimicrobial activities of the total methanol extract and ethyl acetate fraction Methods: The ethyl acetate and n-butanol fractions of Livistona decipiens leaf methanol extract were subjected separately to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1H / 13C NMR). Antioxidant activitywas evaluated by DPPH assay, while evaluation of cytotoxicity was done according to MTT cell viability assay. Antimicrobial activity was done by agar diffusion method. Results: seven compounds were isolated from the ethyl acetate and n-butanol fractions, they were identified for the first time from the plant as apigenin-8-C-β-D-glucopyranoside (Vitexin) (1), Quercetin-6-C-β-D-glucopyranoside (2),apigenin-6,8-di-C-β-D-glucopyranoside (Vicenin II) (3), 6-O-methyl Kaempferol 3-O-glucopyranoside (4), luteolin-3-C-gentiobiosyl (5),luteolin-6-C-β-D-glucopyranoside (Isoorientin) (6) and luteolin-8-C-β-D-glucopyranoside (Orientin) (7). The ethyl acetate fraction has shown moderate activity against Gram positive and Gram negatvie bacteria, also it showed moderate antioxidant activity with IC50 = 23 ± 0.74 µg/ml when compared to ascorbic acid IC50 = 14.2 ± 0.35 µg/ml. Also ethyl acetate extract has shown cytotoxic activity on MCF-7 cells (human breast cancer cell line), HepG-2 (human hepatocellular carcinoma) and HeLa cells (human cervical cancer cell line), whereas, the methanol extract has shown lower activity. Conclusion: Livistona decipienshave potential medicinal value being rich in polyphenolics and beingantioxidant, cytotoxic and antimicrobial drug.
Highlights
Arecaceae, called Palmae, is among the best known and extensively cultivated plant families[1]
Absence of H-8 signal from 1H NMR spectrum of compound 1 has led to conclude that the C-glycosidation must be on C-8. This evidence was confirmed from downfield shift of C-8 to 109.78 (~ + 10 ppm) in 13C NMR spectra of compound 1, the C-glycoside moiety in the structures was confirmed as β-C-glucopyranoside depending on the characteristic up field location of C-1′′ at δ 73.88 ppm and downfield location of both C-5′′ and C-3′′ at δ 82.10 and 79.14 ppm, respectively, with respect to those of Oglycosides
The assignment of all other 13C resonances of compound 1 was achieved by comparison with the corresponding data of structural related compounds[9,14,15,16,17,18,29].According to the above discussed data compound 1 was confirmed as apigenin-8-C-β-Dglucopyranoside (Vitexin) which was isolated for the first time from L. decipiens
Summary
Called Palmae, is among the best known and extensively cultivated plant families[1]. Most work has been carried out on economically important palms such as Phoenix dactylifera, Cocos nucifera and other palms cultivated for their oils[2]. Livistona are widely cultivated as ornamentals in the tropics and subtropics regions[3]. It was found that little work was reported on genus Livistona which showed that plants belonging to this genus are rich in flavonoids, phenolic acids, ceramides, glycerides, lipids and acylglucosylsterols[1,5,6,7,8]. The few phytochemical reports on L. decipiens Becc showed that the lipoidal http://aprh.journals.ekb.eg/ 256
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