Investigation of Antidiabetogenic Effect of the Iodine-Selenium Concentrate in Animals with Chronic Alloxan Diabetes of Varying Severity

Abstract

BACKGROUND: The diabetogenic effect of alloxan is known is determined by ability to stimulate lipid peroxydation processes in B-cells of the pancreas.
 AIM: to investigate of the possible antidiabetic action of long time prolonged of Iodine-Selenium concentrate action in rats with alloxan diabetes.
 METHODS: Reproduction of experimental alloxan diabetes was carried out in rats by a single intravenous injection of alloxan 35-43 mg / kg body weight. The “iodine-selenium” concentrate was administered per os through a tube at the rate of 1.25 ml / 100 g of the concentrate. In experimental animals with mild and heavy diabetes mellitus, the level of glucose in the blood was assessed, products of LPO-AOD; the state of the histostructure of the pancreas and of insulin content in B cells were studied using of aldehyde fuchsin and diethylpseudoisocyanine methods.
 RESULTS: Long time prolonged administration of the “iodine-selenium” concentrate (60 days) to animals with mild experimental diabetes mellitus is accompanied by a significant decrease in blood glucose levels by 1.89 times compared to the control (p ˂ 0.05) and of LPO within normal values as by increase of the level of glutathione peroxidase (GPO) by 2.23 times compared with the initial (p˂ 0.01), by prevention of the development of histological changes in pancreatic islets and a slight decrease of insulin content in B-cells. Under similar experimental conditions in animals with severe alloxan diabetes, the level of glycemia significantly decreased from 20.23 ± 2.15 mmol / l to 12.39 ± 1.52 mmol / l as of the level of diene conjugates of erythrocytes and plasma, as decrease of ketodienes, MDA of plasma and in erythrocytes and primary lipid peroxidation products, while remaining elevated, despite an increase in GPO by 50.0% compared with control (p˂0.05) in the presence of histological changes in the pancreatic islets as in experimental diabetes.
 
 CONCLUSION: The antidiabetic effect of the “iodine-selenium” concentrate in rats with mild alloxan diabetes on the level of glycemia, LPO - AOD and state of the histostructure of the pancreas and the content of deposited insulin in pancreatic B-cells, is probably due to antioxidant effect of selenium to stimulate activity of glutathione blocking lipid peroxide and hydrogen peroxide in alloxan diabetes mellitus.