The metabolic correction of the experimental alloxan diabetes by means of the antioxidant remedies

Abstract

The research concerning the effect of the remedies with the different antioxidant mechanisms on the indices of carbohydrate and lipid metabolism as well as the thiol link of the antioxidant system and the functional detoxication system in modeling conditions of the decompensated alloxan diabetes mellitus have been presented. The research was performed on 105 white nonlinear male-rats with initial body weight (b.w.) 200-230 g, divided into seven groups. Group 1 has consisted of the intact animals. Group 2 (the comparison group) has consisted of the animals suffering from the severe alloxan diabetes mellitus (intraperitoneal administration of alloxan monohydrate at 10 mg/100 g b.w. three times, with an interval of one day, against fasting) without correction. In groups 3-7 diabetes mellitus has also been modeled. The animals of group 3 were fed by the diet with the supplement containing the antioxidant complex with high content of carotenoid substances (1 mg lutein per day, orally for a month before simulations of diabetes mellitus and for a month after). The animals of group 4 have been given the sodium dichloroacetate (15 mg/100g bw) with drinking water for the month before and a month after the modeling of diabetes. The animals of group 5 have been injected with the reduced glutathione (15 mg/100 g of b.w.) daily one week prior to administration of alloxan and until the end of the experiment, the animals of group 6 have been injected with the quercithin (10 mg/100 g of b.w.) and the rats of group 7 have been given the water with the reduced content of deuterium (91 mg/l) instead of the usual drinking water. The development of experimental alloxan diabetes mellitus in rats has been accompanied by the increase in blood plasma of glucose concentration by 6.9-fold, cholesterol and lipoproteins of low density by 66 and 100% respectively. The compensatory activity increase of the antiradical protective enzymes (glutathione peroxidase and glutathione reductase) as well as the concentration decrease of reduced glutathione in erythrocytes by 11% have been revealed. According to the data concerning the level of the molecules with medium and low mass in blood plasma as well as in erythrocytes, the evident endogenous intoxication has taken place. The performed researches have proved the presence of the significant hypoglycemic effect of the usage of sodium dichloroacetate, of the water with the reduced content of deuterium and of quercithin - the glucose concentration in these groups was lower than in the comparison group by 36, 33 and 47% respectively. In the same groups as well as in the group of rats injected with glutathione the hypolipidemic effect of various manifestation degree has been detected, the most evident in animals from group 7 in which all the indices except triglycerides haven't differed from indices of the control group. The usage of various corrective methods has also assisted the adequate functioning of the antioxidant system that has been proved by the normalization of glutathione content except of groups 3 and 6 in which its concentration was lower than the control indices by 25 and 12% respectively. Endogenous intoxication has been significantly lower in groups 4, 5 and 7 in which the level of molecular of medium and low mass in blood plasma was normal and only in erythrocyte mass was increased by 49-74% than in the control group. Thus, the data obtained prove the usage availability of the antioxidant remedies in the complex therapy in patients suffering from the diabetes mellitus. The most interesting is the usage of antioxidants with the indirect mechanism of action, i.e. the water with the reduced content of deuterium and sodium dichloroacetate. Quercithin and the reduced glutathione deserve attention as well, and considering the hypoglycemic effect of the first one and the metabolic effects of glutathione aimed at the functioning maintenance of the non-specific organism resistance, the possibility of the combined usage of both antioxidants should be taken into account.