Abstract
We demonstrated the microtransfer molding of Norland Optical Adhesive 81 (NOA81) thin films. NOA81 nanogrooves and flat thin films were transferred from a flexible polydimethylsiloxane (PDMS) working mold. In the case of nanogrooves, the mold's feature area of 15 × 15 mm2 contains a variety of pattern dimensions in a set of smaller nanogroove fields of a few mm2 each. We demonstrated that at least six microtransfers can be performed from the same PDMS working mold. Within the restriction of our atomic force microscopy measurement technique, nanogroove height varies with 82 ± 11 nm depending on the pattern dimensions of the measured fields. Respective micrographs of two of these fields, i.e., one field designated with narrower grooves (D1000L780, case 1) and the other designated with wider grooves (D1000L230, case 2) but with the same periodicity values, demonstrate faithful transfer of the patterns. The designated pattern dimensions refer to the periodicity (D) and the ridge width (L) in the original design process of the master mold (dimensional units are nm). In addition, neither NOA81 itself (flat films) nor NOA81 nanogroove thin films with a thickness of 1.6 μm deteriorate the imaging quality in optical cell microscopy.
Highlights
To be able to further our knowledge on nanogroove-cell interactions with different materials, we investigate in this paper the fabrication method of Norland Optical Adhesive 81 (NOA81) nanogrooves by microtransfer molding
The high quality in transferring the smallest features by jet-and-flash nanoimprint lithography from the original quartz stamp into nanoresist scaffolds has been jeopardized by applying multiple copies in the process to reach the final NOA81 nanogroove thin films on acceptor substrates, microtransfer molding is successfully demonstrated according to the description given in Sec
By AFM, we demonstrated a quantitative proof of the successful microtransfer molding of the pattern onto an acceptor substrate by providing a well-defined thin film of NOA81 (1.6 μm thickness) using spin-coating onto a plasma treated PDMS working mold
Summary
To be able to further our knowledge on nanogroove-cell interactions with different materials, we investigate in this paper the fabrication method of Norland Optical Adhesive 81 (NOA81) nanogrooves by microtransfer molding.NOA81 (Norland Products Inc., NJ, USA) is a single component adhesive which is applied in liquid form and subsequently cured under ultraviolet (UV) light within a few seconds to minutes at a peak sensitivity around a wavelength of 365 nm. This transparent material was first introduced as a fast bonding agent for optical components and later as a biocompatible alternative for polydimethylsiloxane (PDMS) in microfluidics, cost-effective microchannel fabrication, and cell culture devices, including brain-on-chip (BOC) applications. Microfluidic BOCs have emerged rapidly in the BioMEMS field to advance neurodegenerative disease modeling. the toolbox for clinical relevant organ and disease models relies on innovative micro- and nanofabrication techniques, allowing the mimicry of well-defined, reproducible, and controllable microenvironments for culturing tissues. These in vivo like environments can be designed to exert control over the organization, manipulation, and analysis of the cultured cells. NOA81 (Norland Products Inc., NJ, USA) is a single component adhesive which is applied in liquid form and subsequently cured under ultraviolet (UV) light within a few seconds to minutes at a peak sensitivity around a wavelength of 365 nm.. NOA81 (Norland Products Inc., NJ, USA) is a single component adhesive which is applied in liquid form and subsequently cured under ultraviolet (UV) light within a few seconds to minutes at a peak sensitivity around a wavelength of 365 nm.1 This transparent material was first introduced as a fast bonding agent for optical components and later as a biocompatible alternative for polydimethylsiloxane (PDMS) in microfluidics, cost-effective microchannel fabrication, and cell culture devices, including brain-on-chip (BOC) applications.. The toolbox for clinical relevant organ and disease models relies on innovative micro- and nanofabrication techniques, allowing the mimicry of well-defined, reproducible, and controllable microenvironments for culturing tissues.. Topographical features and material stiffness affect cells’ morphology during the neuronal cell network formation in vitro. in culturing neuronal cells, nanoscale topographies can alter neural cell network architecture, enhancing neuronal differentiation and, advance in vitro neurodegenerative disease models
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