Abstract
Rutin is prone to degradation during processing, highlighting the need for a suitable protein system for its delivery. This study evaluated the effects of pH and concentration ratios on the interaction between rutin and superoxide dismutase (SOD), aiming to develop a suitable non-covalent delivery system. The highest thermal stability was observed at pH 4 with a rutin-to-SOD concentration ratio of 5:1, where the melting temperature (Tm) reached 67.8 °C, and the half-life was 176 min. Infrared spectroscopy and particle size analysis revealed that rutin has minimal impact on the secondary structure of SOD; however, high concentrations of rutin influence the quaternary structure of SOD, leading to aggregation. Molecular docking and molecular dynamics simulations further confirmed that the interaction between rutin and SOD occurs through hydrogen bonding and hydrophobic interactions, with pH altering their binding sites. At pH 5 and pH 6, the digestive stability of the SOD-rutin complex was the highest. This study provides a theoretical basis for the preparation of highly stable SOD-rutin complexes and identifies a new protein system for the stabilization of rutin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
