Abstract
Publisher Summary This chapter discusses a study to analyze amino acid transmitters in the neuromuscular junction of invertebrates. The slow flexor muscle was isolated from the crayfish abdominal muscle together with the innervating nerve fibers. This muscle forms a thin sheet of a few muscle fiber's thick and is a good preparation for the perfusion experiment. The nerve muscle preparation was placed in a small bath and thoroughly washed for 30–60 min. A saline solution of 100 μl in volume was put in the bath and after 10 min, this solution was collected and the concentration of glutamate in the solution was measured by the mass fragmentography using a gas chromatograph–mass spectrometer. It was found that the resting release of glutamate was in average about 20 pmole and the nerve stimulation significantly increased the glutamate content in the solution. The net increase of glutamate release above the background was about 10 pmole at 10 Hz stimulation and 20 pmole at 20 Hz stimulation.
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