Abstract
Integrity of the terminal pCpCpA sequence of tRNA's extracted from yeasts in different growth states The accepting activity of baker's yeast tRNA's for some amino acids, such as proline, lysine or arginine, is smaller when tRNA is extracted from exponentially growing yeast than when tRNA is extracted from yeast in the stationary phase or from yeast whose growth has been blocked by inhibitory substances (tubercidin, 5-fluorouracil, cycloheximide). This difference disappears or becomes smaller when the amino acid fixation test is performed not only in the presence of ATP but also of CTP. This result can be explained by a greater detachment of the terminal pCpCpA triplet in the case of tRNA's extracted from exponentially growing yeast. The significance of our results and their practical use are discussed.
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