Abstract
Interspecies specificity between tRNA and aminoacyl-tRNA synthetases from several sources (soybean, corn, and pea seedlings, carrot root, yeast, Neurospora crassa, Escherichia coli) was tested. Sizeable differences in both the rate and total level of aminoacylation of tRNA occurred in heterologous systems derived from evolutionarily distant species. Conversely, similar relative rates and plateau levels of acylation of leucine and tyrosine tRNA occurred in homologous and heterologous systems derived from closely related organisms such as pea and soybean. Only minor differences in relative rates of aminoacylation occurred in all higher plant crosses.
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