Abstract
The rate of synthesis of DNA in HeLa cultures treated with small doses (0.5 μg/ml) of actinomycin D for 30 min is only depressed for a limited period of time: 6 h after the addition of the drug, DNA and RNA metabolism in the cell recover and quickly reach the control level. Herpes simplex virus-infected cells show the same phenomenon if treated with the antibiotic not later than 1 h after infection. Equilibrium sedimentation experiments in a CsCl gradient show that viral DNA replication is prevented if the drug is added during the first 6 h after infection, or irreversibly blocked if added during the following phase of viral DNA replication. Thus, the recovery of the DNA synthesis is limited to cellular DNA. It is concluded, therefore, that actinomycin D in appropriate doses preferentially inhibits herpes virus DNA replication.
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