Abstract

Electrochemical immunosensors combine the advantages of electrochemical transducers such as low cost, potential for miniaturization, and quantified readout with the selective properties of immunochemical recognition elements. Here we describe a simple, label-free electrochemical immunosensor for the rapid detection of traumatic brain injury (TBI) by quanitifcation of a cytokine interleukin-6 (IL-6) in cerebrospinal fluid (CSF). The sensor system consists of gold interdigitated electrode arrays (IDEAs) that are modified with self-assembled monolayers (SAMs) and IL-6 antibodies (IL-6 mAb) for antigen recognition and capture. Electrode modification was confirmed using surface characterization (FT-IR, fluorescence) and electrochemically via EIS. The sensor performance was evaluated in IL-6 spiked PBS solution: the LOD was determined to be 1.63 pg/mL, with a linear range across 4 orders of magnitude. The sensor exhibits selectivity against non-target cytokines TNF-α and IL-10. The detection of CSF and serum IL-6 using the outlined system will be discussed for use as a point-of-care (POC) diagnostic and prognostic tool for TBI.

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