Integration of microfluidic injection analysis with carbon nanomaterials/gold nanowire arrays-based biosensors for glucose detection

Abstract

Carbon nanotubes (CNTs) and reduced graphene oxide (rGO) nanosheets were utilized to construct glucose biosensors in combination with gold nanowire arrays (AuNWAs), and microfluidic injection analysis driven by gravity force was used to investigate the performances of as-prepared glucose biosensors. The results demonstrated glucose biosensors based on carbon nanomaterials/AuNWAs presented excellent performance at low working potential of –0.2V versus Ag/AgCl (3mol/L KCl), such as high sensitivity, good anti-interference ability and high throughput (45h−1). The glucose biosensor based on glucose oxidase (GOx)–CNT–AuNWAs showed a wide linear range from 100 to 3,000μmol/L with a sensitivity of 4.12μA/cm2 mmol/L. Furthermore, the linear range and sensitivity of GOx–rGO–AuNWAs-based glucose biosensor were 50–4,000μmol/L and 8.59μA/ (cm2 mmol/L), respectively, which were better than those of glucose biosensor based on GOx–CNT–AuNWAs, suggesting rGO nanosheets in combination with AuNWAs being a good platform for the construction of glucose biosensors.