Abstract
We report on a new type of amperometric glucose biosensor that was made by integration of glucose oxidase (GOD) with palladium nanoparticles/reduce graphene oxide (Pd/RGO) nanocomposite. The Pd/RGO was prepared by a one-step reduction method in which the palladium nanoparticles and the reduced graphene oxide (RGO) were simultaneously accomplished from the reduction of dispersed solution of PdCl2 and graphite oxide (GO) with hydrazine. The asprepared nanocomposite exhibits favorable electrocatalytic activities towards the oxidation of H2O2, which makes it a good platform for the construction of the glucose biosensor. The analytical performance of the glucose biosensor is fully evaluated. It shows good analytical properties in terms of a short response time (3 s), high sensitivity (14.1 μA/mM), and low detection limit (0.034 mM). In addition, the effects of pH value, applied potential, electroactive interference and the stability of the biosensor were discussed as well.
Highlights
Though glucose biosensor has been already commercialized for decades, the basic research is still active [1,2,3,4,5]
We report on a new type of amperometric glucose biosensor that was made by integration of glucose oxidase (GOD) with palladium nanoparticles/reduce graphene oxide (Pd/reduced graphene oxide (RGO)) nanocomposite
Due to the outstanding electrocatalytic activities of the Pd/RGO/GCE towards H2O2, an amperometric glucose biosensor was constructed by integration of glucose oxidase (GOD) with the Pd/ORG/GCE, and the evaluation of glucose concentration in aqueous solution by the biosensor was achieved by the catalytic oxidation of hydrogen peroxide
Summary
Though glucose biosensor has been already commercialized for decades, the basic research is still active [1,2,3,4,5]. The side product, hydrogen peroxide, is usually oxidized at conventional solid state electrodes, and the kinetics is very sluggish, high over potentials are often required in order to get a sufficient sensitivities This may give rise to serious interference from some electroactive species (i.e. ascorbate, urate, acetaminophen, etc.) which are usually present in real samples. Reduce graphene oxide (RGO), a flat monolayer of graphite packed into a two-dimensional (2D) honeycomb lattice, has become one of the most novel and exciting stars in material science This inexpensive material has a unique ability to promote fast electron transfer kinetics for a wide range of electroactive species when it is employed as electrode substrate [20,21,22,23,24]. This feature encouraged us to develop a new amperometric glucose biosensor by combination with glucose oxidase
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