Abstract
In this research, a direct, simple and ultrasensitive fiber optic particle plasmon resonance (FOPPR) biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide (AuNP/GO) composite as signal amplification element. To obtain the best analytical performance of the sensor, experimental parameters including the surface concentration of GO on the AuNPs, formation time of the GO, the concentration of the anti-IgG and incubation time of anti-IgG were optimized. The calibration plots displayed a good linear relationship between the sensor response (ΔI/I0) and the logarithm of the analyte concentrations over a linear range from 1.0 × 10−10 to 1.0 × 10−6 g/mL of IgG under the optimum conditions. A limit of detection (LOD) of 0.038 ng/mL for IgG was calculated from the standard calibration curve. The plot has a linear relationship (correlation coefficient, R = 0.9990). The analytical performance of present work’s biosensor was better than that of our previously reported mixed self-assembled monolayer of 11-mercaptoundecanoic acid/6-mercapto-1-hexanol (MUA/MCH = 1:4) method by about three orders of magnitude. The achieved good sensitivity may be attributed to the synergistic effect between GO and AuNPs in this study. In addition, GO could immobilize more antibodies due to the abundant carboxylic groups on its surface. Furthermore, we also demonstrated that the results from this sensor have good reproducibility, with coefficients of variation (CVs) < 8% for IgG. Therefore, the present strategy provides a novel and convenient method for chemical and biochemical quantification and determination.
Highlights
Clinical disease detection requires testing tools for real-time diagnosis and precision medicine, which are helpful in the early detection of disease, effective prevention and treatment, thereby improving the success rate and mortality of patients in clinical monitoring and treatment [1]
We demonstrated a simple, rapid and versatile in situ approach for the fabrication of graphene oxide by using a modification of the Hummers’ method, which does not involve the use of surfactants [24,25]
Au nanoparticles (AuNPs)/graphene oxide (GO)-functionalized sensing various instruments used for preparation of theincluding
Summary
Clinical disease detection requires testing tools for real-time diagnosis and precision medicine, which are helpful in the early detection of disease, effective prevention and treatment, thereby improving the success rate and mortality of patients in clinical monitoring and treatment [1]. Technique for real-time monitoring of heart medicine on cardiac muscle cell function and behavior [2], surface-enhanced Raman scattering (SERS) plus a microfluidic system for detecting prostate-specific antigen (PSA) [3], fluorescence immunoassay (FIA) for detecting biomarkers (adenosine) in the urine of lung cancer patients [4], colorimetric assays for detecting heart disease bioindicator troponin I (cardiac troponin I, cTnI) [5], fluorescent semiconductor quantum dot (QD) for detecting the influenza virus (influenza H1N1 virus) [6], radioimmunoassay (RIA) for detecting insulin in plasma [7] and enzyme-linked immunosorbent assay (ELISA) for detecting carcinoembryonic antigen (CEA) [8] These optical detection techniques have some disadvantages such as high prices, time requirements, the need for specific operators, unstable isotopes, large sizes and difficult field assays. At present, sensing platforms have developed according to the sandwich hybridization detection method and competition method to enhance their sensitivity [14,15,16], but the fabrication procedure is complicated, and the stability requires further enhancement
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