Insect frass from upcycling vegetable by-products with cereals: Effects on the soil properties, plant development and soil invertebrate fitness

Abstract

The use of insects in organic management systems is expanding due to their ability to recycle waste into valuable co-products for agriculture, notably frass, constituted by the insect's excrements, larval exuviae, and remaining undigested feedstock. This study aimed to assess the effects of different application rates of frass (0.16, 0.32, 0.64, 1.28, 2.56 and 5.12%) produced by black soldier fly Hermetia illucens larvae (BSFL) on the survival and reproduction of two non-target invertebrate species, the enchytraeid Enchytraeus crypticus and the collembolan Folsomia candida, and early development of three representative species of crops as onion Allium cepa, turnip Brassica rapa and tomato Solanum lycopersicum. Chemical analyses were conducted to evaluate changes in the soil properties. Results showed that BSFL frass did not impact the invertebrates' survival while significantly enhancing the production of E. crypticus juveniles (after 21 days). F. candida juveniles remained similar (after 28 days). Seed germination decreased at the highest frass rate (5.12%), while the development was promoted at intermediate rates (0.64%–1.28%). The different outcomes may be linked to changes in certain soil parameters, such as the soil pH and electrical conductivity, the soil organic matter, and the availability of nutrients. In summary, frass posed no risk to the tested invertebrate species but may hinder seed germination at high rates, representing a risk for agricultural production. Nevertheless, intermediate rates of BSFL frass promoted plant development, showing potential as a sustainable alternative to conventional fertilizers. Further research is needed to ensure its safe and efficient application in agriculture.