Abstract
To obtain standardized inoculum with constant characteristics for biodegradability test, Actizym powder was used instead of sludge. A predigestion and stabilization step were introduced to prepare “activated Actizym”, before inoculation and addition of test chemical. The inoculum obtained is similar to activated sludge in its microbiological and physical aspects. The reproducibility of the method was prooved and several controls were made using chemicals with different biodegradation rates.
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