Abstract
The binding of Met-tRNA Met m and the initiator tRNA, Met-tRNA Met f, to rabbit reticulocyte ribosomes in the presence of various oligoribonucleotides containing the methionine codon, AUG, was studied in an in vitro system. 1. 1. At low concentrations of Mg 2+, optimum binding of Met-tRNA Met in response to AUG was observed in the concentration range of 1.5–4 M potassium acetate. 2. 2. Triplet specificity between other aminoacyl-tRNAs and their codons was retained under these ionic conditions. 3. 3. Met-tRNA Met f was shown to have a much higher affinity for ribosomes in response to AUG than Met-tRNA Met m. 4. 4. The binding of Met-tRNA Met f was specific for the 40-S ribosomal subunit. 5. 5. Met-tRNA Met f appeared to set the proper reading frame for the specific binding of a second aminoacyl-tRNA molecule to the 40-S ribosomal subunit.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
