Abstract
In the West Siberia there is very little data on the biology of cenopopulations of Gymnadenia conopsea. Habitat destruction and environmental changes, which potentially affected species long-term viability, led to G. conopsea cenopopulations fragmentation and isolation. A detailed study of the morphology variability of the Fragrant orchid representatives was carried out in the cenopopulation in Novosibirsk region. Our results indicate that asymbiotic germination of G. conopsea seeds is difficult to achieve and the species has serious conservation issues. It is the first report when the introduction of G. conopsea in tissue culture was undertaken as the initial stage of germplasm conservation of Siberian cenopopulation. Three modified nutrient media with different growth additives were tested to promote protocorm and seedling formation. The advanced G. conopsea seedlings establishment was obtained in 1/3 Murashige and Skoog (MS) medium, supplemented by 1.0 mgl−1 2-isopentenyladenine (2iP), 0.1 mgl−1 1-Naphthaleneacetic acid (NAA) and 10% coconut water. This study allowed establishing a reliable and reproducible system for the G. conopsea maintenance and conservation ex situ.
Highlights
Our results indicate that asymbiotic germination of G. conopsea seeds is difficult to achieve and the species has serious conservation issues
The advanced G. conopsea seedlings establishment was obtained in 1/3 Murashige and Skoog (MS) medium, supplemented by 1.0 mgl−1 2isopentenyladenine (2iP), 0.1 mgl−1 1-Naphthaleneacetic acid (NAA) and 10% coconut water
A low proportion of immature plants was the characteristic mode in the CP
Summary
Br. occurs all over Europe from Great Britain to the Mediterranean, eastwards to China and Japan [1]. Fragrant orchid (G. conopsea) is a polycarpic, perennial, terrestrial, glabrous herb [2]. G. conopsea assumed to be Least Concern species (LC), according to the IUCN Red List of Threatened Species In Russia the number and size of G. conopsea cenopopulations (CP) have declined markedly during recent decades, mostly due to habitat destruction, agricultural intensification and overgrazing [3]. Many of the Fragrant orchid’s CP are rural and protected by law in several regions of Russian Federation
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call