Abstract
Objective To investigate the inhibitory effect of recombinant LIGHT-Fc gene on the proliferation of human esophageal carcinoma cell line Eca109. Methods LIGHT-Fc expression vector was transfected into human esophageal squamous carcinoma cell line Eca109 by using DOTAP liposomal transfection reagents. The effects of LIGHT-Fc gene on the proliferation of esophageal carcinoma cell line Eca109 in vitro were detected by cell growth curve and MTT assay, Forty-five nude mice were equally divided into Eca109/Wt group, Eca109/neo group and Eca109/LIGHT group. Carcinogenesis and pathological expression of the esophageal carcinoma tissues were observed. Results The expressions of LIGHT receptors were detected in Eca109 cells. The proliferation of Eca109 cells was inhibited after trasfecting LIGHT-Fc gene into Eca109 cells. The numbers of tumors generated in Eca109/Wt group, Eca109/neo group and Eca109/LIGHT group were 12, 11 and 5, with statistical significance between Eca109/LIGHT group and the other two groups （ X^2 = 6. 652, 4. 821, P 〈 0.05 ）. The result of histopathological examination indicated that the tissue necrosis appeared significantly in tumors derived from Eca109/LIGHT cells. Conclusions The growth of esophageal squamous carcinoma cell line Eca109 can be suppressed by LIGHT-Fc gene whether in vitro or in vivo. Key words: Recombinant gene ; Transfection ; Esophageal carcinoma
Published Version
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