Abstract
Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an IC50 of 70.7±1.72 μM at 24 h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.
Highlights
Esophageal cancer (EC) is one kind of high incidence of upper digestive tract cancers worldwide. GLOBOCAN 2008 has reported an estimated 481645 new cases and 406533 deaths occurred for both sexes (Globocan, 2008)
We investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells
Luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation
Summary
Esophageal cancer (EC) is one kind of high incidence of upper digestive tract cancers worldwide. GLOBOCAN 2008 has reported an estimated 481645 new cases and 406533 deaths occurred for both sexes (Globocan, 2008). Luteolin has been shown to possess multiple biological activities such as anti-inflammation, anti-oxidant and anti-cancer (Ashokkumar et al, 2008; Park et al, 2012). Among these activities, the anti-cancer effect of luteolin has been studied in vitro. The vitro experiment of luteolin affected on human esophageal cancer cell line has not been intensely investigated. We will investigated the anti-cancer potential of luteolin on human esophageal carcinoma cell line Eca109 cells and explored the possible molecular mechanisms. Data from our study expand the spectrum of the anti-cancer effect of luteolin and offer valuable clues to the in vivo study , which was necessary to be carried out in future
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