Inhibitory effect of Anacyclus pyrethrum extract on acetylcholinesterase enzyme by invitro methods

Abstract

The present study was conducted to evaluate acetylcholinesterase (AChE) inhibitory effects of Anacyclus pyrethrum. The hexane, chloroform and ethanolic extract of Anacyclus pyrethrum were tested for their invitro anticholinesterase inhibitory effect by spectrophotometeric and by TLC bioassay method. The result revealed that ethanolic extract of Anacyclus pyrethrum showed better anticholinesterase inhibition compared to other extracts. The most active one was found to be ethanolic extract of Anacyclus pyrethrum having IC50, value at 70±1.52 μg/ml. Hexane extract of Anacyclus pyrethrum has not shown any anticholinesterase inhibitory effect. Chloroform extract of Anacyclus pyrethrum was found to have IC50 value at 150±3.68 μg/ml and rivagistmine was found to have IC50 value to be 350±5.95 μg/ml. TLC bioassay is an easier and rapid means for detection of enzyme inhibition. The hexane, chloroform and ethanolic extract of Anacyclus pyrethrum were tested for their anticholinesterase inhibition by TLC bioassay TLC bioassay is an easier and rapid means for detection of enzyme inhibition. The active spots appeared as white spots on yellow background. Ethanolic extract of Anacyclus pyrethrum showed more than one active spots compared to other extracts. The TLC assay also demonstrated AchE inhibitory activity for ethanolic extract of Anacyclus pyrethrum.