Inhibitory effect of Dalbergia latifoia extract on acetylcholinesterase enzyme by invitro methods

Abstract

The present study was conducted to evaluate acetylcholinesterase (AChE) inhibitory effects of Dalbergia latifoia. The hexane, ethylacetate and ethanolic extract of Dalbergia latifoia were tested for their invitro anticholinesterase inhibitory effect by spectrophotometeric and by TLC bioassay method.The result revealed that ethanolic extract of Dalbergia latifoia showed better anticholinesterase inhibition compared to other extracts. The most active one was found to be ethanolic extract of Dalbergia latifoia having IC50 value at 81±1.56 µg/ml. Hexane extract of Dalbergia latifoia has not shown any anticholinesterase inhibitory effect. Ethylacetate extract of Dalbergia latifoia was found to have IC50 value at 625±2.65 µg/ml. TLC bioassay is an easier and rapid means for detection of enzyme inhibition. The hexane, ethylacetate and ethanolic extract of Dalbergia latifoia were tested for their anticholinesterase inhibition by TLC bioassay TLC bioassay is an easier and rapid means for detection of enzyme inhibition. The active spots appearedas white spots on yellow background. Ethanolic extract of Dalbergia latifoia showed more than one active spots compared to other extracts. The TLC assay also demonstrated AchE inhibitory activity for ethanolic extract of Dalbergia latifoia. Keywords: Dalbergia latifoia, acetylcholinesterase, Alzheimer’s disease, TLC bioautography