Abstract
This chapter discusses the inhibition of mitogen-induced blast transformation by male genital components. Human and guinea pig seminal plasma can cause specific and nonspecific inhibition of in vitro cell-mediated immune (CMI) responses. Following experimental or natural sensitization to spermatozoa, a specific antibody and a CMI response occurs. In a study described in the chapter, human peripheral blood lymphocytes were isolated from whole heparinized blood, drawn from normal donors, using Ficoll–Hypaque gradient centrifugation. The cells obtained were used for the microblast transformation and leukocyte inhibition tests procedures. Polyhydroxyalkanoates (PHA)- and spontaneous-induced blast transformation were found to be significantly inhibited by 100 and 400 μg/ml human seminal plasma. The degree of the inhibition of PHA and concanavalin A-induced blast transformation by human spermatozoa is decreased by saturating the spermatozoa active sites with higher amounts of mitogens. Male genital components are immunosuppressive if used in the in vitro CMI systems. These components have antigenic specificity and concurrent suppressive properties, which can mitigate against documentation of immune responses to them.
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