Abstract
Supply of 0.01 to 1.0 mM lead acetate to greening pea(Pisum sativum L.) leaf segments either in the absence or in the presence of inorganic nitrogen lowered total chlorophyll (Chl) content. During a time course study, there was not any appreciable effect of Pb2+ upto 4 h but thereafter Pb inhibited Chl synthesis. While supply of succinate, cysteine dithiothreitol, 5,5-dithio-bis-2-nitrobenzoic acid and NH4C1 had no protective action against Pb2+ toxicity, and glycine, glutamate 2-oxoglutarate, MgCl2, KH2PO4, CaCl2, KC1 protected only partially, reduced glutathione (GSH) could completely overcome the inhibition of Chl biosynthesis by the metal. It is suggested that Pb2+ interferes with Chl biosynthesis through GSH availability
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