Abstract
Fourier-transform infrared attenuated total reflection (ATR) spectroscopy was used to study the effect of volatile anesthetics on fully hydrated dipalmitoylphosphatidylcholine (DPPC) vesicle membranes. The main phase transition was monitored by the change in the C-H 2 asymmetric stretching frequencies of the lipid tails. The surface property was analyzed by the changes in the PO stretching, (CH 3) 3−N + stretching of the hydrophilic head, and CO stretching of the glycerol skeleton. The partial pressures of those agents that decreased the transition temperature 1.0 C° were halothane 0.75, enflurane 1.90 and CCl 4 0.85 kPa. At a 2:1 lipid/anesthetic mole ratio, the polar anesthetics, halothane and enflurane, increased the ratio of (PO stretching band area)/( (CH 3) 3-N + stretching band area) by 26.3% and 21.1%, respectively, whereas apolar CCl 4 increased it 10.5%. The water molecules bound to the PO moiety are apparently replaced by the anesthetic molecules. The deconvoluted CO spectra showed two peaks: free sn-1 that is closer to the lipid core and hydrogen-bonded sn-2 that is closer to the polar head. Addition of halothane and enflurane, but not CCl 4, increased the number of peaks to three. The third peak is free sn-2, formed by disrupting hydrogen-bonding to water. Because the temperature-induced spectral change was limited to C-H 2 stretching at the main phase transition, the effects of anesthetics on the lipid membrane structure are not identical to temperature elevation. Among anesthetics, the effects of apolar and polar molecules on the interfacial properties are different.
Published Version
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