Abstract
1. 1. RNA · DNA hybridization was used to investigate the RNA species stored in unfertilized sea urchin eggs as well as those sequences transcribed on the embryonic genomes soon after fertilization. 2. 2. RNA was extracted from eggs and made radioactive by methylation with [ 3H]dimethyl sulfate. It was found that this RNA contains sequences which will hybridize with at least 3% of the sea urchin genome. 3. 3. RNA was labeled with [ 32P]phosphate during the morula stage of cleavage. In competition-hybridization experiments it was found that 70 % of the 32P-labeled RNA which hybridized was not compted by “maternal” sequences present in the egg. The embryonic genomes of cleavage blastomeres are therefore transcribed to make RNA sequences distinct from those stored in the egg.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
