Influence of Stimforte on the immunologic characteristics of mice against induced immunosuppression

Abstract

Event Abstract Back to Event Influence of Stimforte on the immunologic characteristics of mice against induced immunosuppression Olga V. Lebedinskaya1*, Elena A. Lebedinskaya1, Evgenia V. Fadeeva1, Nelly K. Akhmatova2, Dmitriy G. Maldov2 and Mikhail V. Kiselevsky2 1 Acad. E.A. Wagner Perm State Medical Academy, Russia 2 N.N. Blokhin Russian Cancer Research Center RAMS, Russia The aim of study was the analysis of Stimforte effect on immunological parameters in mice against induced immunosuppression. Investigation was carried out with 80 BALB/c mice there were injected either with cyclophosphane (CP) and Stimforte in different combinations or with physiological solution (control group). The immunophenotype of mononuclear leukocytes (ML) isolated from animal spleen and killer activity against YAK-1 tumor line were defined. Morphological investigations of Romanosvky-Giemsa-stained smears were administrated. CP administered (100 mg/kg) within 3 days evoked immunosuppression that was manifested in nearly 7-fold lowering of ML number in mice spleen as compared with control group. ML population in CP-received animal group was represented by mature lymphocytes, whereas control group demonstrated considerable amount of young lymphoid cells in spleen. Animals against CP introduction exhibited significant decrease in natural killer (NK) and pronounced reduction in their NK-activity. Stimforte injection against CP-induced immunosuppression increased more than by 2-fold the ML content in mice spleen, when immature forms such as pro-lymphocytes were detected. In addition, Stimforte favored the normalization of NK content in spleen ML and their NK activity increment nearly to the initial values. Peripheral blood of animals under study also demonstrated the increase in granulocyte number. Thus, CP resulted in marked immunosuppression manifesting in lowering in total lymphocyte amount in mice peripheral blood and spleen, as well as declining in NK content in lymphocyte population as compared with intact animals. Stimforte administration against cytostatic-induced immunosuppression resulted in significant increase in lymphocyte number and normalization of their immunophenotype and function characteristics. Keywords: Stimforte, induced immunosuppression, cyclophosphane, immunophenotype, mononuclear leukocytes Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Lebedinskaya OV, Lebedinskaya EA, Fadeeva EV, Akhmatova NK, Maldov DG and Kiselevsky MV (2013). Influence of Stimforte on the immunologic characteristics of mice against induced immunosuppression. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00845 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Olga V Lebedinskaya, Acad. E.A. Wagner Perm State Medical Academy, Perm, Russia, lebedinska@mail.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga V Lebedinskaya Elena A Lebedinskaya Evgenia V Fadeeva Nelly K Akhmatova Dmitriy G Maldov Mikhail V Kiselevsky Google Olga V Lebedinskaya Elena A Lebedinskaya Evgenia V Fadeeva Nelly K Akhmatova Dmitriy G Maldov Mikhail V Kiselevsky Google Scholar Olga V Lebedinskaya Elena A Lebedinskaya Evgenia V Fadeeva Nelly K Akhmatova Dmitriy G Maldov Mikhail V Kiselevsky PubMed Olga V Lebedinskaya Elena A Lebedinskaya Evgenia V Fadeeva Nelly K Akhmatova Dmitriy G Maldov Mikhail V Kiselevsky Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.