Fucoidan effect on mononuclear leukocyte immunophenotype and lymphoid organ morphology in mice

Abstract

Event Abstract Back to Event Fucoidan effect on mononuclear leukocyte immunophenotype and lymphoid organ morphology in mice Olga V. Lebedinskaya1*, Elena A. Lebedinskaya1 and Nelly K. Akhmatova2 1 Acad. E.A. Wagner Perm State Medical Academy, Russia 2 I.I. Mechnikov Institute for Vaccines and Sera, RAMS, Russia The aim of investigation was to study the fucoidan effect on immunophenotype characteristics of spleen mononuclear leukocytes (ML) and on structure and cell composition of mice lymphoid organs. Fucoidan was administrated to CBA mice in a dose of 200 mg/kg of body weight. Subset structure of lymphocytes isolated from spleen was examined with flow cytometry. ML immunophenotype assay was carried out under the administration of two types of preparation - fucoidan japonica and cidcorideus to splenocyte cultures in a dose of 10 μg/ml. Paraffin sections of lymphoid organs were stained by histological and histochemical methods. Intraperitoneal fucoidan injection evoked 1,5-2-fold increment in natural killers-T cells, by 2-6-fold - the amount of T- and B-lymphocytes, and by 1,5-fold -the number of cytotoxic lymphocytes. Morphological analysis revealed the expansion of thymus lobe cortex, enlargement of periarterial muffs area, lymphoid nodes frequently merging in conglomerates in spleen. Following the introduction each of two fucoidan preparations to ML mice spleen cultures there were observed similar changes in splenocyte: statistically significant increase in amount of NK, NKT-cells, activated T-helpers, B-lymphocytes, and decrease in CD3+ and CD8+ cells. Pronounced TLR expression by cell surface was noted. It was demonstrated that fucoidan modulated the effector activity of innate and adaptive immunity and resulted in lymphoid cell proliferation in primary and secondary lymphoid organs. Its ability to promote Toll-like receptor expression by ML surface seems to be promising for its use in immunotherapy. This work was supported by RFBR grant №11-04-96037r_ural_a and administrative body of Perm Region. Keywords: fucoidan, spleen mononuclear leukocytes, Lymphoid organs, Immunotherapy, morphological analysis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Lebedinskaya OV, Lebedinskaya EA and Akhmatova NK (2013). Fucoidan effect on mononuclear leukocyte immunophenotype and lymphoid organ morphology in mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00842 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Olga V Lebedinskaya, Acad. E.A. Wagner Perm State Medical Academy, Perm, Russia, lebedinska@mail.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga V Lebedinskaya Elena A Lebedinskaya Nelly K Akhmatova Google Olga V Lebedinskaya Elena A Lebedinskaya Nelly K Akhmatova Google Scholar Olga V Lebedinskaya Elena A Lebedinskaya Nelly K Akhmatova PubMed Olga V Lebedinskaya Elena A Lebedinskaya Nelly K Akhmatova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.