A NK complex-linked locus restricts the spread of Herpes simplex virus type 1 (HSV-1) in the central nervous system (CNS) of C57BL/6 mice

Abstract

Event Abstract Back to Event A NK complex-linked locus restricts the spread of Herpes simplex virus type 1 (HSV-1) in the central nervous system (CNS) of C57BL/6 mice Lorne F. Kastrukoff1*, Allen Lau1, Fumio Takei2, Anthony Scalzo3 and Francis R. Carbone4 1 University of British Columbia, Medicine, Canada 2 British Columbia Cancer Research Center, Terry Fox Laboratory, Canada 3 Lions Eye Institute, Immunology, Australia 4 University of Melbourne, Microbiology and Immunoloy, Australia Natural resistance to mortality in mice infected with Herpes simplex virus type 1 (HSV-1) is mouse strain dependent and an autosomal dominant trait. In mice infected with virus via the oral mucosa, HSV-1 spreads throughout the central nervous system (CNS) of susceptible strains but is restricted to the brainstem of resistant mice. In resistant C57BL/6 (BL/6) mice, viral restriction is mediated by natural killer (NK) cells and CD8+ T-lymphocytes. We combined mendelian analysis, studies of congenic and intra-NKC recombinant mice, along with antibody depleted mice to further examine the restriction of viral spread in the brains of BL/6 mice. We report a NK complex-linked genetic locus, Hrl2, whose alleles determine the restriction of viral spread in the CNS. Mendelian analysis determined that restriction of viral spread is a dominant trait and consistent with a single gene effect. Studies with congenic mice determined that the locus maps to the NKC on chromosome 6 but is separate from previously identified loci; Hrl1 and Rhs1. Studies with intra-NKC recombinants determined that the locus maps to a segment between CD69 and D6Wum34. Studies with antibody depleted mice determined the effect of this locus is mediated by NK1.1 expressing cells. A NK complex-linked locus (Hrl2) restricts the spread of HSV-1 in the CNS of BL/6 mice however the precise mode of action of the locus remains unknown. Acknowledgements This work has been funded by the Canadian Institutes of Health Research Keywords: NK complex linked locus, Herpes simplex virus type 1, Central Nervous System, C57BL/6J mice, NK cells Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Kastrukoff LF, Lau A, Takei F, Scalzo A and Carbone FR (2013). A NK complex-linked locus restricts the spread of Herpes simplex virus type 1 (HSV-1) in the central nervous system (CNS) of C57BL/6 mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00050 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Lorne F Kastrukoff, University of British Columbia, Medicine, Vancouver, British Columbia, V6T 1Z3, Canada, lornefk@mail.ubc.ca Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lorne F Kastrukoff Allen Lau Fumio Takei Anthony Scalzo Francis R Carbone Google Lorne F Kastrukoff Allen Lau Fumio Takei Anthony Scalzo Francis R Carbone Google Scholar Lorne F Kastrukoff Allen Lau Fumio Takei Anthony Scalzo Francis R Carbone PubMed Lorne F Kastrukoff Allen Lau Fumio Takei Anthony Scalzo Francis R Carbone Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.