Abstract
The induction and growth of quinoa’s callus depend on several factors, including the culture medium and the nature of the growth hormone and its dose. In effect, the best callogenesis rates were obtained with the media MS and B5 with respect to the media WHITE and KNOP the callogenesis is too low or zero. The best combination used was 0.2 BA + 2.4 D give well-developed callus. To obtain water-stress resistant cell lines, the effect of water stress induced by polyethylene glycol (P.E.G 6000) on the growth, osmotic potential and metabolic parameter of Chenopodium quinoa callus was studied. Applied water stress showed a reduction in the growth of stressed callus compared to the control. The presence of PEG in the culture medium caused a decrease in the content of fresh matter as well as the dry matter content compared to the control. Water stress also significantly affected the water parameters of calluses. The chlorophyll a, b and carotenoids content decreased, but this decrease is not too pronounced.
Highlights
IntroductionThe in vitro culture is a very recent technique since it was developed only at the beginning of this century by GAUTHRET [1], who was among the first to cultivate tissues, coming from cambial cells of different trees (poplar, maple, willow), on agar medium under aseptic conditions
The in vitro culture is a very recent technique since it was developed only at the beginning of this century by GAUTHRET [1], who was among the first to cultivate tissues, coming from cambial cells of different trees, on agar medium under aseptic conditions
Plants can be regenerated from callus tissues of various explants by dedifferentiation prompted by exogenous growth regulators
Summary
The in vitro culture is a very recent technique since it was developed only at the beginning of this century by GAUTHRET [1], who was among the first to cultivate tissues, coming from cambial cells of different trees (poplar, maple, willow), on agar medium under aseptic conditions. Under the term "in vitro culture" is hiding much diversified fields and techniques, which strongly developed and specialized since thirty years. In vitro culture methods apply to a very diversified material (protoplasts, tissues, organs, whole plants), to achieve oriented objectives, either towards research or towards industrial production [2]. Plants can be regenerated from callus tissues of various explants by dedifferentiation prompted by exogenous growth regulators.
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