Abstract
The current study was carried out to elucidate a reproducible protocol to develop plants directly from leaf and internodes to facilitate the genetic transformation in Coccinia cordifolia (L.) Cogn., a medicinal plant of the cucurbitaceae family. In vitro grown leaf and internodes were used, which were collected for regenerated shoots from field-grown nodal segments that were sterilized by 0.1% HgCl2 treatment for 6 minutes. The nodal segment cultured on BAP, Kn and BAP, NAA combination where 1.5 mg/L BAP solitary supplement and augmented supplement with 0.1 mg/L NAA was most effective as 80% shoots regenerated with 4.0±0.37 and 2.7±0.45 shoots per culture, respectively. Collected leaf and internode responded 90% at 1.5 mg/L BAP + 0.1 mg/L NAA fortified full-strength MS medium. The highest number of shoots also regenerated in the same combination which were 8.1 ± 0.30 and 10.2±0.40, respectively and found internode as the best explant for direct organogenesis. For root induction, half strength of MS medium supplemented with 0.1 mg/L IBA was found most effective. The highest number of roots regenerated per shoot (6.8±0.10) and root length (2.8±0.20). The successful acclimatization of the in vitro (80%) grown plantlets proved the validity of the developed protocol of using biotechnological techniques for improving the plant.
Highlights
IntroductionOrganogenesis is the formation of adventitious shoots (callogenesis), roots (rhizogenensis) from the cells in a rigidly controlled culture medium with exogenous hormone
Organogenesis is the formation of adventitious shoots, roots from the cells in a rigidly controlled culture medium with exogenous hormone
The current study was carried out to elucidate a reproducible protocol to develop plants directly from leaf and internodes to facilitate the genetic transformation in Coccinia cordifolia (L.) Cogn., a medicinal plant of the cucurbitaceae family
Summary
Organogenesis is the formation of adventitious shoots (callogenesis), roots (rhizogenensis) from the cells in a rigidly controlled culture medium with exogenous hormone. This technique is generally achieved by developing meristemoids (a group of meristematic cells) that initiate primordium formation. Shoot regeneration from callus is not preferable as it causes the loss of plantlets identity in an uncontrolled manner from mother plants (Alam et al, 2019). Direct organogenesis is most preferable to produce mass propagation with true-to-type strains and facilitates genetic transformation with minimal possibilities of somaclonal variation or alteration of the target plant genome in the propagated plantlets (Shibli & Smith, 1996; Verma et al., 2021). Direct organogenesis diversifies the plants’ genetic constituents in contrast to micropropagation which declines the genetic diversity (Yang et al, 2012)
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