INFLUENCE OF ISCHEMICAL-REPERFERUAL SYNDROME ON ACTIVATION OF THE LIPID PEROXIDATION SYSTEM IN THE LIVER TISSUES IN EXPERIMENT

Abstract

The purpose of the study. The leading mechanism of damage after ischemia / reperfusion is the overproduction of reactive oxygen species. This process leads to the formation of a number of products of lipid peroxidation (LPO).In order to clarify the development of the process of lipid peroxidation, the study of diene conjugates is important. They are the primary products of lipid peroxidation and relate to toxic metabolites that have a damaging effect on lipoproteins, proteins, enzymes and nucleic acids.The above mechanisms are actively occurring in victims with combined trauma and reperfusion injury, and at the same time justifies the need to study changes in the LPO system in such victims in order to correct their treatment.The aim of the work is to study the effect of ischemic-reperfusion syndrome on changes in the lipid peroxidation system of the liver tissue in an experiment in animals with a combined injury of the abdominal organs.Material and methods. In the experiment, 80 mature white male Wistar rats weighing 190-220 g were used.Animals were divided into control and 3 experimental groups (8 animals in each group). In the animals of the first experimental group the development of ischemic-reperfusion syndrome was studied. In the second group under study, a closed injury of the abdominal organs and hypovolemic shock were modeled. In the third group, damages of the first two groups were combined. The control animals were given anesthesia, without the formation of any injuries.In the liver homogenate, the level of diene conjugates, triene conjugates, and malondaldehyde was examined in a day, three, and seven days after injury modeling.Animals of the experimental groups were removed from the experiment in the conditions of thiopental sodium anesthesia by the method of total bleeding from the heart.Results. Analysis of the obtained results showed that the imposition of hemostatic harnesses for a period of 2:00 causes a significant activation of the POL system in the liver tissue, even after seven days, the indicators of MDA, DC and TC did not return to normal values. Comparing the research groups with each other, they have found that the content of DC in the second group was statistically higher than in the first and less than in the third one. In DG-3, the indicator DU is statistically greater than in DG - 1 and DG - 2 in all control points of the experimental study.In DG - level 3, MDA grew up to seven days: starting from the first day it increased 9.4 times (p <0.001), after three days it increased 15.7 times (p <0.001) and seven days later it increased 18.2 times ( p <0.001) compared to benchmarks.The previously mentioned shows the prospect of further research in the context of the correction of systemic changes that occur following the ischemic period.Findings. The ischemic-reperfusion syndrome in the model of a combined injury of the abdominal organs leads to the development of systemic changes, which is indirectly indicated by a significant increase in the performance of the POL system.