Abstract
In the present paper, the influence of AC electric field on charge accumulation in the measuring cell of a flow-based system for atomic force microscope (AFM) based fishing is considered. As a flow-based system, a device for AFM-based fishing of low-abundant proteins has been employed. The charge is generated in a femtomolar albumin solution during its flowing through an injector of this system. We have demonstrated that application of sinusoidal electric field (100 V, 50 Hz frequency) stimulates charge accumulation in the measuring cell upon the input of protein solution at 38°C. Taking this effect into account is of importance for the development of novel highly sensitive flow-based proteomic and diagnostic systems employing AFM-based fishing, for modeling the influence of electric field on hemodynamics, and also for the development of specified theoretical models describing physicochemical properties of water and aqueous solutions.
Highlights
The present study considers the influence of AC electric field on a charge accumulation in the measuring cell of a flow-based AFM-fishing system for the detection of low-abundant proteins
The charge is generated in a femtomolar (10-15 M) albumin solution, which is flowing through the injector of this system
It has been demonstrated that the sinusoidal electric field (100 V, 50 Hz) stimulates the accumulation of charge in the measuring cell upon inflow of protein solution at 38◦С temperature
Summary
The present study considers the influence of AC electric field on a charge accumulation in the measuring cell of a flow-based AFM-fishing system for the detection of low-abundant proteins.
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