Industrial Propagation of <i>Coffea</i> SP. by Bioreactor Technique

Abstract

Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture of coffea plant in area of over 40.000ha in South of Vietnam. Good quality of seedlings have a big demand. Using bioreactor technique is the first method to resolve the lack of seedlings. Stem, leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supplemented with MS+ BA (0.1mg/l) + 2iP (2mg/l) + IBA (2mg/l). Somatic cell suspension was created and proliferated on medium MS + kinetin (1mg/l) + BA (0.1mg/l) + 2iP (2mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS + kinetin (1mg/l) + BA (4mg/l). In vitro shoots of coffee were regeneration on the medium MS + kinetin (1mg/l) + BA (4mg/l) + Adenin sulfate (40mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26±2°C, 11.1-22.2μmol/m²/s, and 30rpm. Micropropagation of <i>coffea</i> sp. by bioreactor technique was set up to produce 25,260 plantlets per liter of somatic embryogenesis suspension.