Industrial Propagation of Rhynchostylis Sp. By Bioreactor Technique

Abstract

Rhyncostylis sp. propagation by multi-shoot system induction from meristem culture in invitro. It take more labor, energy, large area, and high cost. Plant cell technology is effective way for micropropagation in bioreactor. Protocorm like bodies were used as planting materials. Somatic embryo callus were initiated on medium MS + IAA (1.0 mg/l). Somatic cell suspension were cultured for initiation and for proliferation on medium MS + 2.4D (0.5 mg/l) + kinetin (1 mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension were cultured in bioreactor for initiation and proliferation on the medium MS + NAA (0,5 mg/l) + 2.4D (1 mg/l). Embryogenic suspension was stimulated on the medium MS + NAA (0.3 mg/l) + BA (0.3 mg/l). In vitro shoots of Rhynchostylis were plating and regeneration on the medium MS + NAA (0,1 mg/l) + BA (0,5 mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26+2oC, 11,1-22,2 μmol/m2/s, and 30 rpm. Micropropagation of Rhynchostylis sp. by bioreactor technique was set up to produce 6,800 plantlets per one liter of somatic embryogenesis suspension