Industrial Propagation of Cymbidium Sp. by Bioreactor Technique

Abstract

Micropropagation of orchid plant for conservation and development is needing. Traditional propagation of Cymbidium sp. requires energy cost, many labor, large area for growing, slow growth and development, and high cost input. It is a need to find new effective ways for in vitro propagation, and plant cell technology via bioreactor techniques effort the demands. Protocorm like bodies were used as planting materials. Somatic embryo callus were initiated on medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l). Somatic cell suspension were cultured for initiation and for proliferation. on medium MS + BA (0.1mg/l) supplemented with 2.4D (1 mg/l) and NAA (1 mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension were cultured in bioreactor for initiation and proliferation on the medium MS + BA (0.1mg/l) supplemented with 2.4D (1 mg/l) or NAA (1 mg/l). Embryogenic suspension was stimulated on the medium MS + BA (0.5 mg/l) + NAA (0.1 mg/l). In vitro shoots of Cymbidium sp. were regeneration on the medium MS + BA (0.1 mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26+2oC, 11,1-22,2 μmol/m2/s, and 30 rpm. Micropropagation of Cymbidium sp. by bioreactor technique was set up to produce 5,600 platlets per one liter of somatic embryogenesis suspension