Industrial Propagation of <i>Chukrasia Tabularis</i> A. Fuss by Bioreactor Technique

Abstract

<i>Background:</i> <i>Chukrasia Tabularis</i> A. Fuss is a valuable woody plant and difficult in reproduction in nature. <i>Objectives:</i> There are a needs to conserve and develop for re-forest.<i> Method:</i> Manipulation of tissue culture via bioreactor techniques was the effective new way to resolve re-forest. Stems and leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium MS + BA (2mg/l) + PVP (500 mg/l) supplemented with 2,4D (2mg/l). Somatic cell suspension was cultured for initiation and for proliferation. On medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). Embryogenic suspension was stimulated on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of <i>Chukrasia Tabularis</i> a. Fuss were regeneration on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with TDZ (0,5mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m²/s, and 30rpm.<i> Results:</i> Micropropagation of <i>Chukrasia Tabularis</i> a. Fuss by bioreactor technique was set up to produce 4,680 plantlets per one liter of somatic embryogenesis suspension <i>Conclusion:</i> a process of rapid industrial multiplication of woody plants by bioreactor technology has been built.