Abstract
Objective To investigate the effect of dendritic cells (DC) and natural killer (NK) cells on the human telomerase reverse transcriptase (hTERT) mRNA in human melanoma cell line A375 in vitro.Methods A co-culture system was established by transwell culture system,in which DC and NK cells obtained from peripheral blood of healthy donors were added to lower chamber for 24 h separately or together.Then A375 cells was co-cultured in upper chamber of the transwell for 24 h,48 h and 72 h.A375 cells were collected and hTERT mRNA were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) assay.Results DC and NK cells reduced the expression of hTERT mRNA in A375 cells separately or together (DC/NK).However,the reduction of hTERT mRNA expression in DC/NK group was more apparently than that in DC or NK group.Furthermore,DC/NK group reduced the hTERT mRNA expression in a timedependent manner.Conclusion This is the first time to demonstrate that DC and NK cells have an indirect influence on the hTERT mRNA expression in human melanoma cell line A375,and there is an synergetic effect between DC and NK.The immune microenvironment created by interaction between DC and NK has a stronger inhibitory effect. Key words: Dendritic cells; Natural killer cells; Telomerase reverse transcriptase; Melanoma
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