Expression of human telomerase reverse transcriptase mRNA in colorectal cancer patients and its clinical significance

Abstract

Objective To investigate the expression of human telomerase reverse transcriptase (hTERT) mRNA in colorectal cancer patients, and to explore its clinical significance. Methods The clinical data of 80 patients with colorectal cancer who received treatment in our hospital from February 2014 to August 2015 were retrospectively analyzed. At the same time, the clinical data of 80 healthy adults were selected as control. To observe the expression of hTERT mRNA in colorectal tissue of two groups of patients, and to compare the expression of hTERT mRNA in colorectal tissue of colorectal cancer patients with different clinicopathological features, and to analyze the factors influencing the expression of hTERT mRNA in colorectal cancer patients. Results The positive expression rate of hTERT mRNA in colorectal tissue of colorectal cancer patients was 87.50%, which was significantly higher than that in normal control group (6.25%). Abdominal lymph node metastasis, histological type and tube adenocarcinoma and TNM stage Ⅲ+ Ⅳ, the degree of differentiation for poorly differentiated colorectal cancer colorectal tissue the positive expression rate of hTERT mRNA was higher (χ2=4.068, 4.898, 8.447, 6.154; P=0.044, 0.027, 0.004, 0.013), different age, gender, colorectal tissues of patients with colorectal cancer hTERT mRNA expression of tumor size and location was not significant difference (χ2=2.593, 0.258, 0.878, 0.152, 1.755; P=0.107, 0.612, 0.349, 0.696, 0.185). Abdominal lymph node metastasis and histological type were the risk factors of hTERT mRNA positive rate in patients with colorectal cancer. The odds ratio (OR) values were 4.115 and 3.985, respectively. Conclusion The expression of hTERT mRNA in colorectal tissue of patients with colorectal cancer was higher, and the existence of patients with lymph node metastasis, tumor stage and histological type are closely related, as an important index for clinical screening of colorectal cancer. Key words: Colorectal cancer; Human telomerase reverse transcriptase; Logistic regression analysis