Abstract
A cell-free system capable of carrying out amino acid incorporation into peptides was developed from exponentially growing cultures of Pseudomonas aeruginosa. The amino acid incorporation activity required an ATP energy generating system, Mg 2+, NH 4 +, tRNA and an amino acid mixture for maximum activity. Ribonuclease, puromycin and chloramphenicol inhibited protein synthesis. N-Formylmethionyl-tRNA was synthesized with a P. aeruginosa enzyme system, methionine, P. aeruginosa tRNA, formate and tetrahydrofolic acid. N-formylmethionine was incorporated into peptides by the P. aeruginosa cell-free system. These results indicate that P. aeruginosa extracts contain methionyl-tRNA transformylase and the complete system for the incorporation of N-formylmethionine into peptides.
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