Abstract
Biomaterials may be used as treatment of great abdominal wall defects to avoid tension during repair. In the present research we intended to investigate incorporation type by host tissue of membranes of microbial cellulose (MC), produced by the bacteria Zoogloea sp., and of polytetrafluoroethylene (ePTFE) in abdominal wall defects of rats. Sixty male rats Wistar, anesthetized by ketamine (5mg/100g) and xylazine (2mg/100g), were submitted to a rectangular excision (2 x 3cm) of the abdominal wall, including fascia, muscles and peritoneum and further treated with implants of microbial cellulose (MC Group - 30 animals) or expanded polytetrafluoroethylene ( ePTFE Group- 30 animals). Each group was subdivided in 14th DPO, 28th DPO and 60th DPO Subgroups. Incorporation of biomaterials was observed by wrapping and infiltration by host tissue. It has been found that wrapping associated to infiltration of host connective tissue in implants of ePTFE were present in 100% of the observed samples, and this may be responsible for increase resistance to traction. Inversely, wrapping without host tissue infiltration was seen in 100% of examined specimens of MC implants. Wrapping and host tissue infiltration is seen only in ePTFE implants.
Highlights
In Veterinary Medicine, few experimental reports for clinical applications were found on the use of biocelulose produced by Acetobacter xylinum such as; cuff protection in the reconstruction of peripheral nerves[2], healing of experimental wounds in mammary teats of bovine[3], experimental teguments wounds in equine[4] and swine[5], prophylaxis of the occurrence of membrane after laminectomy in dogs[6] and healing of experimental incisional lesions of the cornea in canine[7]
The animals were distributed in two groups: Microbial Cellulose Group (MC Group): composed of 30 animals that were submitted to a muscle-aponeurotic defect on ventral wall of abdomen and treated with membrane of microbial cellulose; Expanded Polytetrafuoroethylene Group: composed of 30 animals that were submitted to a muscle-aponeurotic defect on ventral wall of abdomen and treated with membrane of expanded polytetrafluoroethylene; Each group was subdivided in three Subgroups of 10 rats, in agreement with the postoperative day (POD) observation, being denominated of 14th POD Subgroup, 28th POD Subgroup and 60th POD Subgroup
Microbial Cellulose Group The observation of the specimens stained by H-E and Tricromic of Masson, in14th DPO, 28th DPO and 60th DPO Subgroups of MC Group revealed a type of incorporation characterized by presence of wrapping without infiltration in implants in 100% of the sample readings (Table 1, Figure 1)
Summary
In Veterinary Medicine, few experimental reports for clinical applications were found on the use of biocelulose produced by Acetobacter xylinum such as; cuff protection in the reconstruction of peripheral nerves[2], healing of experimental wounds in mammary teats of bovine[3], experimental teguments wounds in equine[4] and swine[5], prophylaxis of the occurrence of membrane after laminectomy in dogs[6] and healing of experimental incisional lesions of the cornea in canine[7]. The first clinical application of membranes of biocelulose produced by microorganism Zoogloea sp., in gross state, was done in UFRPE to treat natural cutaneous wounds of dogs. The authors, possibly took advantage of the beneficial properties of sugar, main constituent of the wrapping of gross membranes (sugar-cane molasses), on wound healing. Studies of biocompatibility and citotoxity of the microbial cellulose produced by Zoogloea sp. were previously done, and authorize the accomplishment of experimental research for clinical application . 10,11,12,13
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