Biomechanical evaluation of microbial cellulose (Zoogloea sp.) and expanded polytetrafluoroethylene membranes as implants in repair of produced abdominal wall defects in rats

Suyiene Cordeiro Falcão,Joaquim Evêncio Neto,Antônio Roberto De Barros Coelho

doi:10.1590/s0102-86502008000200012

Suyiene Cordeiro Falcão, Joaquim Evêncio Neto + Show 1 more

Open Access

https://doi.org/10.1590/s0102-86502008000200012

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Abstract

To evaluate the Load of Rupture of implants of membranes of microbial cellulose (Zoogloea sp.) and extended polytetrafuoroethylene in sharp defects of abdominal wall of rats. Sixty Wistar male rats, with a mean weight of 437,7 g +/- 40,9, anesthetized by a mixture of ketamine (5mg/100g) and xylazine (2mg/100g), were submitted to a rectangular (2 x 3 cm) excision of the abdominal wall, including fascia, muscle and peritoneum, and treated with membranes of microbial cellulose (MC) (MC Group- 30 animals) or extended polytetrafluoroethylene (ePTFE) (ePTFE Group- 30 animals). Each group was subdivided in 14th POD, 28th POD and 60th POD Subgroups. Under anesthesia, animals were submitted to euthanasia at 14th POD, 28th POD and 60th POD for evaluation of Load of Rupture. Load of Rupture levels were significantly elevated (p<0, 05) among 14th, 28th and 60th postoperative days from each Group. When compared between groups, values of Load of Rupture were significantly larger (p<0, 05) in ePTFE Group than in MC Group. Resistance to strength at implant/host interface was more pronounced in PTFEe Group than in MC Group.

Highlights

IntroductionIn some circumstances the repair can be affected by the great distance among the edges of the defect or by the lack of tissue with characteristics for an appropriate approach
The best method for the treatment of muscleaponeurotics defects of the abdominal wall is the approach, without tension, of fibromuscular structures of the own patient’s tissue.in some circumstances the repair can be affected by the great distance among the edges of the defect or by the lack of tissue with characteristics for an appropriate approach
Top clamp was attached to a Digital Dynamometer®g and the bottom clamp was connected to Comparative analysis accomplished among the averages of the values of the Load of Rupture obtained in the animals of 14th POD, 28th POD and 60th POD Subgroups of microbial cellulose (MC) Group are shown in graphic form (Figure 6)

Summary

Introduction

In some circumstances the repair can be affected by the great distance among the edges of the defect or by the lack of tissue with characteristics for an appropriate approach. In these cases, synthetic and biological prostheses, or even muscular grafts, vascularized or free, can be used for reconstruction[1]. Important examples include products for temporary skin substitution and tissue replacement. These activities have been accompanied by the isolation of new bacterial strain producing biocellulose[3]

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