Abstract
ABSTRACT The aim of this work was to determine the tenacity of tortoise viruses under natural conditions. Three viruses isolated from Hermann's tortoises, Testudo hermanni, or spur-thighed tortoises, Testudo graeca, were used as test agents: a chelonian herpesvirus, isolate 4295/7R/95, an iridovirus, isolate CH8/96, and a picomavirus-like virus “x”, isolate TGT1A/96. Each virus was adsorbed onto an electropositively charged membrane filter which was sandwiched between two polycarbonate membranes with pores. The sandwiched membranes were stored in drinking water and lake water at 4°C (39°F), room temperature (between 20 and 27°C (68 and 81°F) and 37°C (99°F) in the laboratory, and in soil outside in the early spring and in the summer. The remaining virus was eluted after defined intervals and quantified by virus titration. The reduction of infectious virus was very small over weeks at 4°C (39°F) in drinking and lake water. Three log10 units of virus were inactivated in about one to eight weeks at room temp...
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